A Rapid Modification of the Cephalin Cholesterol Flocculation Test.

نویسندگان

A A ROSENBERG

L G EIMANN

J O'LEARY

چکیده

‘THE LENGTHY INCUBATION PERIOD and the subjective interpretation of degree of flocculation ai’e undesirable characteristics of the Hanger cephalin cholesterol flocculatiomi test (1). Several workers (2-4) have reported modifications either to shorten test time or to determine extent of flocculation mnstrumemitally. Inadequate sensitivity has been noted in some of these technics as well as interference frommi chyle in serum. A cephalin cholesterol flocculation procedure is outlined which incorporates a relatively short imicubation period and spectrophotometric measurement of the flocculated antigemi particles to accomplish both rapidity amid objectivity. The new method has distinct advantages: Results of tests are reportable the same day the specimen is submitted. Specimens received late in the week can be conveniently processed since the 1or 2-day incubation period is eliminated. (‘hyle amid bilirubin which are removed in the process cannot interfere with instrumental measurements of resuspended antigen particles.

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